CST, 80974S, NeuN (D4G4O) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying NeuN. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, NeuN (D4G4O) Mouse Chimeric Monoclonal Antibody (CST #80974) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity NeuN (D4G4O) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total NeuN protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification This recombinant chimeric antibody is engineered from NeuN (D4G4O) XP Rabbit mAb #24307 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, NeuN (D4G4O) XP Rabbit mAb #24307, is produced by immunizing animals with a recombinant protein corresponding to human NeuN protein. Background Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). Alternate Names FLJ56884; FLJ58356; fox-1 homolog C; Fox-3; FOX3; hCG_1776007; hexaribonucleotide binding protein 3; HRNBP3; NEUN; NeuN antigen; neuronal nuclei; Neuronal nuclei antigen; RBFOX3; RFOX3; RNA binding fox-1 homolog 3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a