CST, 80981S, DHODH Antibody

Polyclonal Antibody for studying DHODH. Validated for Western Blotting. Highly specific and rigorously validated in-house, DHODH Antibody (CST #80981) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DHODH Antibody recognizes endogenous levels of total DHODH protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly301 of human DHODH protein. Antibodies are purified by peptide affinity chromatography. Background Dihydroorotate dehydrogenase (DHODH) catalyzes the conversion of dihydroorotate to orotate in pyrimidine biosynthesis. DHODH was shown to be a metabolic regulator of myeloid differentiation in AML models. Inhibition of this enzyme triggers myeloid differentiation, decreases leukemic cell burden, and prolongs survival (1). In addition, research studies show that pyrimidine biosynthesis mediated by DHODH is critical for tumorigenesis. Cancer cells deleted of fail to form tumors, suggesting DHODH as a potential target in cancer therapy (2). Further studies also indicate that loss of DHODH diminishes tumor growth (3). Alternate Names DHOdehase; DHODH; dihydroorotate dehydrogenase; dihydroorotate dehydrogenase (quinone); Dihydroorotate dehydrogenase (quinone), mitochondrial; Dihydroorotate dehydrogenase, mitochondrial; Dihydroorotate oxidase; human complement of yeast URA1; POADS; PYRD; URA1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 SOURCE: Rabbit