CST, 81007S, STAP2 Antibody

Polyclonal Antibody for studying STAP2. Validated for Western Blotting. Highly specific and rigorously validated in-house, STAP2 Antibody (CST #81007) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity STAP2 Antibody recognizes endogenous levels of total STAP2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human STAP2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background STAP2 (signal-transducing adaptor protein 2, BRK substrate, BKS) is an adaptor protein expressed in various tissues and cell lines (1). The protein sequence of STAP2 contains a PH domain, an SH2 like domain, a proline rich region, and a YXXQ motif for interaction with various signaling molecules. (2). In the immune system, STAP2 plays important roles in cytokine signaling, macrophage activation, T cell migration, and motility via interaction with, and regulation of, signaling molecules such as STAT3, STAT5, IKKα/β, CSF1R, VAV, FAK, and Fas ligand. In leukemia, prostate cancer, breast cancer, and melanoma, STAP2 has been shown to promote tumor growth and metastasis via stabilization and activation of signaling molecules, including BCR/ABL, EGFR, BRK, STATs, and tyrosinase (3-6). Alternate Names BKS; Breast tumor kinase substrate; brk kinase substrate; BRK substrate; FLJ20234; signal transducing adaptor family member 2; Signal-transducing adaptor protein 2; signal-transducing adaptor protein-2; STAP-2; STAP2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50, 55 SOURCE: Rabbit