CST, 81191SF, Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Diablo/Smac. Validated for WB,IHC,IF,IF,F. Highly specific and rigorously validated in-house, Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #81191) is ready to ship. Product Usage Information This product is the carrier free version of product #15108. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15108 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Smac/Diablo protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Smac/Diablo protein. Background Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6). Alternate Names 0610041G12Rik; DBLOH; DFNA64; DIABLO; diablo homolog (Drosophila); Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMAC3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG