CST, 81197T, LAMP2A (F1A5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP2A (F1A5A) Rabbit Monoclonal Antibody (CST #81197) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity LAMP2A (F1A5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP2A protein. This antibody specifically detects the LAMP2A isoform (P13473-1) but not the LAMP2B (P13473-2) or LAMP2C (P13473-3) isoforms. Species reactivity by immunofluorescence is mouse only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LAMP2A protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Oxidation-induced activation of chaperone-mediated autophagy (CMA) is attained through transcriptional up-regulation of LAMP2A (7). Alternate Names CD107 antigen-like family member B; CD107b; DND; LAMP-2; LAMP2; LAMPB; LGP-96; LGP110; lysosomal associated membrane protein 2; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100-130 Source/Isotype: Rabbit IgG