CST, 81221T, CYFIP1 (E6X1U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CYFIP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CYFIP1 (E6X1U) Rabbit Monoclonal Antibody (CST #81221) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CYFIP1 (E6X1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total CYFIP1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro527 of human CYFIP1 protein. Background Cytoplasmic FMR1-interacting protein 1 (CYFIP1) is a component of the CYFIP1/EIF4E/FMR1 complex which mediates translational repression by binding to the mRNA cap (1). CYFIP1 also plays a role in neuronal axonal growth dynamics by binding to the WAVE complex to regulate remodeling of actin filaments (2). Mutations in the gene encoding CYFIP1 has been linked to multiple neural development and psychiatric disorders, including autism spectrum disorder and schizophrenia (3-6). The specific mechanism by which CYFIP1, which is enriched in synapses, contributes to these neurological diseases is unknown, but may involve regulating the balance of synaptic excitation and inhibition to maintain neuronal circuit homeostasis during development and in mature brains (7). Alternate Names CYFIP1; CYFP1; cytoplasmic FMR1 interacting protein 1; Cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; FLJ45151; KIAA0068; p140sra-1; selective hybridizing clone; SHYC; Specifically Rac1-associated protein 1; Sra-1; SRA1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG