CST, 81254T, PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PVR. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody (CST #81254) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31235 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD155 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn188 of human CD155 protein. Background Poliovirus receptor (PVR), also known as CD155 and nectin-like 5 (Necl-5), is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR expression may play a role in the invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6). Alternate Names CD155; FLJ25946; HVED; NECL-5; NECL5; nectin-like 5; Nectin-like protein 5; Poliovirus receptor; PVR; PVR cell adhesion molecule; PVS; TAGE4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG