CST, 8126S, CDC73 (D38E12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDC73. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CDC73 (D38E12) Rabbit Monoclonal Antibody (CST #8126) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CDC73 (D38E12) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDC73 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg407 of human CDC73 protein. Background CDC73 (HPRT2) is a putative tumor suppressor protein thought to bind RNA polymerase II to help inhibit cell cycle progression (1,2). Commonly referred to as parafibromin, CDC73 is expressed in endocrine tissues, kidney, heart, and skeletal muscle and is localized to both nuclear and cytoplasmic compartments (3). CDC73 acts as a Wnt signaling regulator as it binds the carboxy-terminal region of β-catenin (4). Mutations in the corresponding gene cause an endocrine disorder known as hyperparathyroidism 2, which is characterized by hypercalcemia, bone resorption, and the development of jaw tumors (5). Alternate Names C1orf28; CDC73; cell division cycle 73; cell division cycle 73 Paf1/RNA polymerase II complex component-like protein; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae); Cell division cycle protein 73 homolog; Familial isolated hyperparathyroidism; FIHP; FLJ23316; HPTJT; HRPT1; HRPT2; Hyperparathyroidism 2 protein; HYX; Paf1/RNA polymerase II complex component; Parafibromin Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG