CST, 8127S, Rab10 (D36C4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rab10. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rab10 (D36C4) Rabbit Monoclonal Antibody (CST #8127) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57398 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rab10 (D36C4) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab10 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab10 protein. Background Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Mss4, myosin V (Va, Vb and Vc) and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosomes and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for insulin-stimulated GLUT4 translocation in adipocytes (7,8). Alternate Names GTP-binding protein RAB10; RAB10; RAB10, member RAS oncogene family; ras-related GTP-binding protein; Ras-related protein Rab-10 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG