CST, 81309SF, TACI/TNFRSF13B/CD267 (E3P6X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TNFRSF13B mouse. Validated for WB,IF,IF,F,F. Highly specific and rigorously validated in-house, TACI/TNFRSF13B/CD267 (E3P6X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #81309) is ready to ship. Product Usage Information This product is the carrier free version of product #93005. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93005 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TACI/TNFRSF13B/CD267 (E3P6X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TACI/TNFRSF13B/CD267 protein. This antibody is predicted to recognize mulitple isoforms of TACI/TNFRSF13B/CD267. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse TACI/TNFRSF13B/CD267 protein. Background Transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) and cluster of differentiation 267 (CD267), is a single-pass type III membrane receptor that is highly expressed on the surface of mature innate-like B cells, such as marginal zone and B-1 B cells in mice, and memory B cells in humans (1-3). TACI/TNFRSF13B/CD267 is also expressed on the surface of activated T cells (4), while monocytes and dendritic cells express lower levels of TACI/TNFRSF13B/CD267 primarily intracellularly (5,6). TACI/TNFRSF13B/CD267 is the receptor for soluble ligands BAFF/TNFSF13B and APRIL/TNFSF13 (7,8). Upon ligation, TACI/TNFRSF13B/CD267 recruits tumor necrosis factor receptor-associated factor (TRAF) family members and calcium signal-modulating cyclophilin ligand (CAMLG) in order to activate NFAT, NF-κB, and AP-1 transcriptional programs (9-11). TACI/TNFRSF13B/CD267 is necessary for T cell-independent type II and type I responses (1,12), negative regulation of B cell and T follicular helper cell numbers (13,14), and promotion of class switch recombination in B cells (15). Mutations and aberrant regulation of TACI/TNFRSF13B/CD267 has been associated with cancer, autoimmune diseases, and immunodeficiency disorders (16-18). Multiple isoforms produced by alternative splicing have been identified. Alternate Names 1200009E08Rik; CD267; OTTMUSP00000006011; Taci; Tnfrsf13b; TR13B; transmembrane activator and calcium-modulating and cyclophilin ligand interactor; Transmembrane activator and CAML interactor; Tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13b Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 20-30 Source/Isotype: Rabbit IgG