CST, 8131T, Phospho-PZR (Tyr241) (D6F9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PZR (Tyr241) phosphate. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PZR (Tyr241) (D6F9) Rabbit Monoclonal Antibody (CST #8131) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-PZR (Tyr241) (D6F9) Rabbit Monoclonal Antibody recognizes endogenous levels of PZR protein only when phosphorylated at Tyr241. Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr241 of human PZR protein. Background PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8). Upon Con A treatment or H O treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9). Alternate Names FLJ21047; immunoglobulin family transmembrane protein; MPZL1; MPZL1b; myelin protein zero like 1; myelin protein zero-like 1; Myelin protein zero-like protein 1; protein zero related; Protein zero-related; PZR; PZR1b; PZRa; PZRb Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 30-50 Source/Isotype: Rabbit IgG