CST, 81375SF, COL1A1 (E8F4L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying COL1A1. Validated for WB,IHC,IHC,IF,IF,ELISA+. Highly specific and rigorously validated in-house, COL1A1 (E8F4L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #81375) is ready to ship. Product Usage Information This product is the carrier free version of product #72026. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 72026 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity COL1A1 (E8F4L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total COL1A1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1196 of human COL1A1 protein. Background Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex composed of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and post-translational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11). Alternate Names Alpha-1 type I collagen; alpha1(I) procollagen; CAFYD; CO1A1; COL1A1; collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen I; collagen of skin, tendon and bone, alpha-1 chain; collagen type I; collagen type I alpha 1 chain; collagen, type I, alpha 1; EDSARTH1; EDSC; OI1; OI2; OI3; OI4; pro-alpha-1 collagen type 1; type I collagen; type I proalpha 1; type I procollagen alpha 1 chain Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG