CST, 8141S, UCHL3 (D25E6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UCHL3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, UCHL3 (D25E6) Rabbit Monoclonal Antibody (CST #8141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UCHL3 (D25E6) Rabbit Monoclonal Antibody recognizes endogenous levels of total UCHL3 protein. This antibody does not cross-react with either UCHL1 or BAP1 and is not predicted to cross-react with UCHL5. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UCHL3 protein. Background Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all posses a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed (3,4). Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. In particular, UCHL3 functions as a Ub hydrolase involved in the processing of both Ub precursors and ubiquitinated substrates, generating free monomeric Ub. This is accomplished through the ability of UCHL3 to recognize and hydrolyze isopeptide bonds at the C-terminal glycine of either Ub or NEDD8 (5-7). Recent functional studies have identified UCH-L3 as a critical regulator of adipogenesis through its ability to promote IGF-IR and insulin receptor signaling (8). Furthermore, UCHL3 has been shown to promote deubiquitination, recycling, and cell surface expression of the epithelial sodium channel (9). Alternate Names testicular tissue protein Li 221; ubiquitin C-terminal hydrolase L3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3; UCHL3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG