CST, 8144S, PDLIM2 Antibody

Polyclonal Antibody for studying PDLIM2. Validated for Western Blotting. Highly specific and rigorously validated in-house, PDLIM2 Antibody (CST #8144) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PDLIM2 Antibody recognizes endogenous levels of total PDLIM2 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly255 of human PDLIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PDLIM2, also known as Mystique, contains an amino-terminal PDZ domain and a carboxy-terminal LIM domain. PDLIM2 was orginally found to be associated with cytoskeletal proteins in epithelial cells to promote cell attachment and migration (1,2). Subsequent studies have shown that PDLIM2 can also inhibit NF-κB activity by acting as a nuclear ubiquitin E3 ligase for p65 (3). PDLIM2 is suppressed in cancer cell lines by DNA methylation (4,5). Expression of PDLIM2 can inhibit anchorage-independent growth and tumor formation. Alternate Names FLJ34715; MYSTIQUE; PDLI2; PDLIM2; PDZ and LIM domain 2; PDZ and LIM domain 2 (mystique); PDZ and LIM domain protein 2; PDZ-LIM protein mystique; SLIM Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit