CST, 81454S, VSV-G Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, VSV-G Tag Antibody (CST #81454) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity VSV-G Tag Antibody detects recombinant proteins containing the VSV-G epitope tag. This antibody recognizes the VSV-G tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects a non-specific band of unknown origin at 65 kDa. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the vesicular stomatitis virus glycoprotein epitope (YTDIEMNRLGK). Antibodies are purified by peptide affinity chromatography. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The VSV-G tag is derived from an epitope from the C-terminal region of vesicular stomatitis virus glycoprotein (1). VSV-G is widely used both as an epitope tag in expression vectors and for pseudotyping lentiviral vectors due to the stability and broad host range of the resulting pseudotypes (2,3). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit