CST, 8145S, LAMTOR2/ROBLD3 (D7C10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMTOR2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMTOR2/ROBLD3 (D7C10) Rabbit Monoclonal Antibody (CST #8145) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LAMTOR2/ROBLD3 (D7C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMTOR2/ROBLD3 protein. This antibody may cross-react with LAMTOR1/C11orf59 and LAMTOR3/MAPKSP1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu31 of human LAMTOR2/ROBLD3 protein. Background mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5). Alternate Names ENDAP; endosomal adaptor protein p14; HSPC003; LAMTOR2; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; late endosomal/lysosomal adaptor, MAPK and MTOR activator 2; late endosomal/lysosomal Mp1-interacting protein; LTOR2; MAPBP-interacting protein; MAPBPIP; MAPKSP1 adaptor protein; MAPKSP1AP; mitogen-activated protein-binding protein-interacting protein; mitogen-activated protein-binding protein-interacting protein (MAPBPIP); P14; Ragulator complex protein LAMTOR2; Ragulator2; roadblock domain containing 3; roadblock domain-containing protein 3; ROBLD3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG