CST, 81479S, Hexokinase II (F9Q4T) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3086)

Monoclonal Antibody for studying Hexokinase 2. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Hexokinase II (F9Q4T) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3086) (CST #81479) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Hexokinase II (F9Q4T) Rabbit mAb (InTraSeq™ 3' Conjugate 3086) recognizes endogenous levels of total hexokinase II protein. This antibody does not cross-react with hexokinase I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hexokinase II protein. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names DKFZp686M1669; hexokinase 2; Hexokinase type II; Hexokinase-2; hexokinase-2, muscle; Hexokinase-B; HK II; HK2; HKII; HXK2; Muscle form hexokinase Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG