CST, 81493S, EOMES (D8D1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EOMES. Validated for WB,IF,F,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, EOMES (D8D1R) Rabbit Monoclonal Antibody (CST #81493) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Plus Sonication Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Chromatin IP: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 71103 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN Specificity / Sensitivity EOMES (D8D1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total EOMES protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro180 of human EOMES protein. Background The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis, and the differentiation of certain T cell subsets. Embryos from EOMES knockout mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knockout mice show defects in development of a specific population of neural progenitors known as intermediate-stage progenitor cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role in neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8 T cells (6). Expression of EOMES is induced in CD8 T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7). Alternate Names EOMES; eomesodermin; Eomesodermin homolog; eomesodermin homolog (Xenopus laevis); t box, brain, 2; T-box brain protein 2; T-brain-2; TBR-2; TBR2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75, 85 Source/Isotype: Rabbit IgG