CST, 8152S, GFAP (GA5) Mouse Monoclonal Antibody (Alexa Fluor® 594 Conjugate)

Monoclonal Antibody for studying GFAP. Validated for Immunofluorescence (Frozen),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, GFAP (GA5) Mouse Monoclonal Antibody (Alexa Fluor^® 594 Conjugate) (CST #8152) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GFAP (GA5) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord. Background The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3). Alternate Names ALXDRD; FLJ45472; GFAP; Glial fibrillary acidic protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1