CST, 81533S, PAF15 (D8E2Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAF15. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PAF15 (D8E2Y) Rabbit Monoclonal Antibody (CST #81533) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:150 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity PAF15 (D8E2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAF15 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PAF15 protein. Background Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). PCNA associated factor (PAF15) interacts with PCNA to recruit DNA replicative polymerase. In response to DNA damage, PCNA recruits a low fidelity DNA polymerase to allow bypass of lesions that would otherwise stall DNA replication. This form of DNA damage tolerance is called translesion synthesis (TLS), and is regulated in part by PAF15 (2,3). Ubiquitination of PAF15 at lysines 15 and 24 regulates its interactions with PCNA and DNA polymerase (3). PAF15 is overexpressed in human cancers, and research studies show a correlation between PAF15 expression and poor prognosis (3,4). Alternate Names HCV NS5A-transactivated protein 9; Hepatitis C virus NS5A-transactivated protein 9; KIAA0101; L5; NS5ATP9; OEATC; OEATC-1; OEATC1; Overexpressed in anaplastic thyroid carcinoma 1; p15(PAF); p15/PAF; p15PAF; PAF; PAF15; PCLAF; PCNA clamp associated factor; PCNA-associated factor; PCNA-associated factor of 15 kDa; PCNA-clamp-associated factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG