CST, 81557S, CUTL1 Antibody

Polyclonal Antibody for studying CUTL1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CUTL1 Antibody (CST #81557) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CUTL1 Antibody recognizes endogenous levels of total CUTL1 protein. This antibody recognizes the p200 isoform (the full-length) and is not expected to recognize the p110 and p75 isoforms. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln639 of human CUTL1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background CUTL1 (Cut-like 1), also known as CUX1 (Cut homeobox 1) (CUX1), is a transcription factor that has been implicated in many cellular processes in different tissues, such as cell migration, neuronal differentiation, and DNA repair (1-5). CUTL1 expression and activities are altered in cancer. Research studies have shown the CUTL1 gene to be a frequent target of loss-of-heterozygocity in various cancers (6,7). On the other hand, CUTL1 expression is elevated in many cancers and is associated with shorter disease-free survival (8). These accumulating evidence suggest that decreased CUTL1 expression promote tumor initiation and increased CUTL1 expression facilitates tumor progression (9). While full-length CUTL1 is about 200 kDa (p200), short forms p110 and p75 can also be generated by proteolytic processing and alternative transcription initiation site, respectively (10, 11). Alternate Names CASP; CCAAT displacement protein; CDP; CDP/Cut; CDP1; Clox; COY1; cut homeobox; cut homolog; cut like homeobox 1; cut-like 1, CCAAT displacement protein; cut-like homeobox 1; CUTL1; CUX; Cux/CDP; CUX1; CUX1 gene Alternatively Spliced Product; GDDI; golgi integral membrane protein 6; GOLIM6; Homeobox protein cut-like 1; Homeobox protein cux-1; mutant CUX1; Nbla10317; p100; p110; p200; p75; protein CASP; putative protein product of Nbla10317 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 SOURCE: Rabbit