CST, 81559S, MCP-1 (E8Y7P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MCP-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MCP-1 (E8Y7P) Rabbit Monoclonal Antibody (CST #81559) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MCP-1 (E8Y7P) Rabbit Monoclonal Antibody recognizes endogenous levels of total MCP-1 protein. A cross-reactive band of unknown origin is observed around 35 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MCP-1 protein. Background Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G protein-coupled receptor CCR2 (9). Alternate Names C-C motif chemokine 2; C-C motif chemokine ligand 2; CCL2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2; SMC-CF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13-15 Source/Isotype: Rabbit IgG