CST, 81563SF, Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FcgR2b mouse. Validated for WB,IHC,IHC,F. Highly specific and rigorously validated in-house, Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #81563) is ready to ship. Product Usage Information This product is the carrier free version of product #96397. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 96397 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FcγRIIB protein. Please note that non-specific signal was observed in kidney tubules by IHC. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro284 of mouse FcγRIIB protein. Background FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5,6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7). Alternate Names AI528646; CD32; F630109E10Rik; Fc gamma receptor IIB; Fc gamma RIIB; Fc receptor, IgG, low affinity IIb; Fc-gamma RII; Fc-gamma-RII; Fc-gamma-RIIB; Fc[g]; Fc[g]RII; Fcg; Fcgamm; FcgammaRIIB; Fcgr; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-; Fcr-2; Fcr-3; FcRII; IgG Fc receptor II beta; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-1; Ly-17; Ly-m2; Ly-m20; LyM-; LyM-1; Lymphocyte antigen 17; OTTMUSP00000024374; OTTMUSP00000024375 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG