CST, 81569S, MCT4/SLC16A3 (F2K6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MCT4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MCT4/SLC16A3 (F2K6A) Rabbit Monoclonal Antibody (CST #81569) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MCT4/SLC16A3 (F2K6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total MCT4/SLC16A3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MCT4/SLC16A3 protein. Background MCT4, also known as MOT4 or SLC16A3, is a multi-pass transmembrane monocarboxylate transporter (MCT) protein encoded by the gene (1,2). MCT4 is reported to be expressed in white (glycolytic) muscle cells (3,4), astrocytes (5), activated macrophages (6), and cancer cells (2). The primary role of MCT4 is lactate exportation, which is adapted to the release of lactate from glycolytic cells (8,9). In the tumor microenvironment, MCT4 mediated lactate secretion promotes M2 macrophage polarization and suppresses T cell function (10). MCT4 inhibition increases the efficacy of immune checkpoint blockade and is a potential target to reverse tumor resistance to immunotherapy (11,12). Alternate Names MCT 3; MCT 4; MCT-3; MCT-4; MCT3; MCT4; MGC138472; MGC138474; Monocarboxylate transporter 3; Monocarboxylate transporter 4; MOT4; SLC16A3; solute carrier family 16 (monocarboxylate transporter), member 3; solute carrier family 16 (monocarboxylic acid transporters), member 3; Solute carrier family 16 member 3; solute carrier family 16, member 3; solute carrier family 16, member 3 (monocarboxylic acid transporter 4) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG