CST, 8157T, Oct-1 (D7B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Oct-1. Validated for WB,IP,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Oct-1 (D7B6) Rabbit Monoclonal Antibody (CST #8157) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq Specificity / Sensitivity Oct-1 (D7B6) Rabbit Monoclonal Antibody recognizes endogenous levels of total Oct-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human Oct-1 protein. Background Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6). Alternate Names NF-A1; Oct-1; oct-1B; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1; Octamer-binding transcription factor-1; OTF-1; OTF1; PO2F1; POU class 2 homeobox 1; POU domain, class 2, transcription factor 1; POU2F1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG