CST, 8162S, Sec23A Antibody

Polyclonal Antibody for studying Sec23A. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Sec23A Antibody (CST #8162) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Sec23A Antibody recognizes endogenous levels of total Sec23A protein. Based on sequence homology, this antibody may cross-react with Sec23B. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe717 of human Sec23A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background There are two isoforms of Sec23 protein: Sec23A and Sec23B. Both isoforms have been shown in the Sec23/24 complex, which is a component of COPII coat (1). COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (2). COPII formation is initiated through binding of the activated G protein, Sar1, to the Sec23/24 complex to form a prebudding complex, which directly binds target molecules (2-4). The prebudding complex further recruits Sec13/31 to form mature COPII coat (5,6). In addition to being a COPII component, Sec23 has also been shown to interact with p125 and Sec16 at the transitional ER; these interactions are important for regulation of the COPII transportation function (7,8). Alternate Names CLSD; hSec23A; MGC26267; Protein transport protein Sec23A; SC23A; Sec23 homolog A (S. cerevisiae); Sec23 homolog A, coat complex II component; Sec23 homolog A, COPII coat complex component; SEC23-related protein A; SEC23A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 82 SOURCE: Rabbit