CST, 81662T, CREST (E3P4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SS18L1. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, CREST (E3P4F) Rabbit Monoclonal Antibody (CST #81662) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity CREST (E3P4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CREST protein. This antibody does not cross-react with SS18 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly168 of human CREST protein. Background Calcium-responsive transactivator (CREST), also known as SS18L1, is a calcium-dependent neuronal BAF family member expressed in the developing brain (1-4). CREST recruits CBP to nuclear bodies in order to regulate cortical and hippocampal dendrite development (1,5). The activity of CREST is aided by the ATPase of the BAF complex, Brg1, which upon calcium influx, will release a phospho-Rb/HDAC1 repressive complex from neuronal genes (4). CREST mutations have been described in ALS. The protein contains a prion-like domain, associates with the ALS protein FUS, forms aggregates, and is included in paraspeckles (6-8). CREST is highly homologous to SS18 and can similarly form fusions with SSX proteins in synovial sarcomas (9,10). Alternate Names Calcium-responsive transactivator; CREST; KIAA0693; LP2261; MGC26711; MGC78386; SS18-like protein 1; SS18L1; SS18L1 subunit of BAF chromatin remodeling complex; SS18L1, nBAF chromatin remodeling complex subunit; synovial sarcoma translocation gene on chromosome 18-like 1; SYT homolog 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG