CST, 81681T, MRCK alpha (E2S5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MRCKa. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MRCK alpha (E2S5I) Rabbit Monoclonal Antibody (CST #81681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MRCK alpha (E2S5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total MRCKα protein. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with MRCKβ or MRCKγ. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1728 of human MRCKα protein. Background CDC42BPA/MRCK (Myotonic dystrophy kinase-related CDC42-binding kinase) comprises a family of serine/threonine kinases that function as downstream effectors of the small GTPase, CDC42. Three MRCK family members have been identified: MRCKα, MRCKβ, and MRCKγ. MRCK family kinases have some substrates in common with the Rho-associated kinases, ROCK1 and ROCK2, including Myosin Light Chain 2 (MLC2). Signaling through both ROCK and MRCK kinase families regulates actin dynamics, cell migration, invasion, and metastasis (1,2). Simultaneous inhibition of both ROCK and MRCK kinases prevents migration and invasion of human cancer cell lines (3). Small molecule inhibitors targeting the MRCK family are being investigated as potential treatments for glioblastoma and skin cancer (4,5). Alternate Names CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha (DMPK-like); CDC42 binding protein kinase beta; CDC42 binidng protein kinase beta; CDC42-binding protein kinase alpha; CDC42-binding protein kinase alpha (DMPK-like); CDC42BPA; DKFZp686L1738; DKFZp686P1738; DMPK-like alpha; FLJ23347; KIAA0451; MRCK; MRCK alpha; MRCKA; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; myotonic dystrophy kinase-related CDC42-binding protein kinase alpha; Myotonic dystrophy protein kinase-like alpha; PK428; ser-thr protein kinase PK428; ser-thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine-protein kinase MRCK alpha Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG