CST, 81693S, CEP55 (D1L4H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CEP55. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CEP55 (D1L4H) Rabbit Monoclonal Antibody (CST #81693) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CEP55 (D1L4H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEP55 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val459 of human CEP55 protein. Background The 55 kDa centrosomal protein (CEP55) is a widely expressed centrosome and midbody-associated protein that regulates cytokinesis, including completion of the final step during cytokinesis known as abscission (1,2). CEP55 activity during abscission is negatively regulated by p53 through Polo-like kinase 1 (3,4). The breast and ovarian cancer DNA repair protein BRCA2 interacts with CEP55 and plays a regulatory role during abscission (5). Research studies demonstrate that CEP55 is also involved in the regulation of Akt signaling, autophagy, and may be a biomarker in human cancer (reviewed in 6). The correlated overexpression of CEP55, the transcription factor FoxM1, and the HELLS helicase is seen in head and neck squamous cell carcinoma (7,8). Additional studies demonstrate that CEP55 expression regulates cell proliferation in gastric carcinoma (9). Alternate Names C10orf3; cancer/testis antigen 111; centrosomal protein 55; centrosomal protein 55kDa; Centrosomal protein of 55 kDa; CEP55; CT 111; CT111; FLJ10540; MARCH; Up-regulated in colon cancer 6; URCC6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG