CST, 81694S, Brachyury (D2Z3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Brachyury. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Brachyury (D2Z3J) Rabbit Monoclonal Antibody (CST #81694) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79925 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Brachyury (D2Z3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total Brachyury protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro256 of human Brachyury protein. Background Brachyury protein, encoded by the gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6). Alternate Names BRAC; Brachyury; Brachyury protein; MGC104817; OTTHUMP00000017588; Protein T; SAVA; T; T brachyury; T brachyury transcription factor; T brachyury-like; T-box transcription factor T; T, brachyury homolog; T, brachyury homolog (mouse); TBXT; TFT; transcription factor T Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG