CST, 81703S, COL7A1 (F4Q8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COL7A1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, COL7A1 (F4Q8A) Rabbit Monoclonal Antibody (CST #81703) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity COL7A1 (F4Q8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL7A1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus region of human COL7A1 protein. Background Type VII collagen is a collagen family protein encoded by the gene. Collagen VII is formed by intertwining three COL7A1 chains to function as an anchoring fibril (1). The anchoring fibril is localized in the basement membrane and crucial for dermal-epidermal basement membrane adhesion through the interaction of the N-terminal NC1 domain of COL7A1 with collagen IV, laminin 332, and collagen I (2-5). Mutations of prevent type VII collagen secretion or interaction with other ECM molecules, lead to anchoring fibril deficiencies in the dermal-epidermal basement membrane, and result in skin blistering (Dystrophic Epidermolysis Bullosa) and poor wound healing (6-8). Type VII collagen deficiency also results in the oncogenesis of cutaneous squamous cell carcinoma through increasing TGF-β signaling and abnormal angiogenesis (9,10). Alternate Names CO7A1; COL7A1; Collagen alpha-1(VII) chain; collagen type VII alpha 1 chain; collagen VII, alpha-1 polypeptide; collagen, type VII, alpha 1; EBD1; EBDCT; EBR1; LC collagen; long chain collagen; Long-chain collagen; NDNC8 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 330 Source/Isotype: Rabbit IgG