CST, 8186S, FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXA2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody (CST #8186) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 38775 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxA2/HNF3β protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly138 of human FoxA2/HNF3β protein. Background Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and, therefore, increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5). Alternate Names forkhead box A2; Forkhead box protein A2; FOXA2; hepatic nuclear factor-3-beta; Hepatocyte nuclear factor 3-beta; hepatocyte nuclear factor 3, beta; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF-3B; TCF3B; Transcription factor 3B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG