CST, 81944T, Aldolase C (E7I4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Aldolase C. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Aldolase C (E7I4X) Rabbit Monoclonal Antibody (CST #81944) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Aldolase C (E7I4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total fructose bisphosphate aldolase C protein. This antibody does not cross-react with fructose bisphosphate aldolase A or fructose bisphosphate aldolase B proteins. This antibody detects a non-specific band of unknown origin at 75 kDa in mouse lung tissue extracts. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn90 of human aldolase C protein. Background Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma (1). Furthermore, investigators have shown that changes in aldolase B gene expression levels have been observed in certain patients with this primary tumor (2,3). Aldolase C is primarily expressed in the central nervous system (4). Studies have shown increased detection of serum aldolase C following traumatic brain injury (TBI) and mild TBI, showing potential for aldolase C as a biomarker for TBI (5,6). Alternate Names ALDC; ALDOC; aldolase 3; aldolase C, fructose-bisphosphate; aldolase, fructose-bisphosphate C; Brain-type aldolase; epididymis secretory sperm binding protein; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; Fructose-bisphosphate aldolase C Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG