CST, 8194T, Bub3 (D8G6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bub3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Bub3 (D8G6) Rabbit Monoclonal Antibody (CST #8194) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bub3 (D8G6) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bub3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human Bub3 protein. Background The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7). Alternate Names BUB3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; Mitotic checkpoint protein BUB3; testicular tissue protein Li 27 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG