CST, 82103S, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Magnetic Bead Conjugate)

Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Magnetic Bead Conjugate) (CST #82103) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in PBS Buffer (pH 7.2), 0.1% Tween ® 20. Store at 4°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (Magnetic Bead Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. This antibody conjugate recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG