CST, 82108S, PDE4A (E5P9A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDE4A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PDE4A (E5P9A) Rabbit Monoclonal Antibody (CST #82108) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PDE4A (E5P9A) Rabbit Monoclonal Antibody recognizes endogenous levels of total PDE4A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu703 of human PDE4A protein. Background Phosphodiesterase (PDE) superfamily enzymes catalyze the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. The PDE superfamily includes 11 subfamilies (PDE1-PDE11) in mammals (1). These enzymes function as important positive and negative regulators of cellular response, including regulation of insulin secretion, heart function, erectile function, and inflammation (2-5). The PDE4 subfamily consists of PDE4A, PDE4B, PDE4C, and PDE4D isoforms, and are cAMP-specific enzymes expressed in T cells, monocytes, macrophages, dendritic cells, neutrophils, and eosinophils (6). PDE4 expression is induced by epithelial damage, allergen sensitization, and microbial invasion (7). Inhibition of PDE4 subfamily members leads to the elevation of intracellular cAMP and modulates the inflammatory response (8). The isoform, PDE4A, is being investigated as a therapeutic target for various inflammatory conditions such as asthma, inflammatory bowel disease (IBD), and rheumatic arthritis (RA), and as an immune checkpoint inhibitor for multiple suppressive factors (9,10). Alternate Names cAMP-specific 3',5'-cyclic phosphodiesterase 4A; cAMP-specific phosphodiesterase; DPDE2; PDE4; PDE46; PDE4A; phosphodiesterase 4A; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila); phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG