CST, 82131S, Rabbit (DA1E) IgG Isotype Control F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, Rabbit (DA1E) IgG Isotype Control F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) (CST #82131) is ready to ship. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Rabbit (DA1E) IgG Isotype Control F(ab') Background Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Human F receptors exhibit strong cross-reactivity with rabbit IgG, and are expressed at high levels on cells of myeloid lineage (e.g., monocytes and myeloid-derived cell lines). Fluorescent F(ab') fragment conjugates are ideal for use in flow cytometry assays on cells containing F receptors, and eliminate the need for an F blocking step prior to antibody incubation. Cell Signaling Technology employs strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence. Specification Concentration: 100 ug/ml SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG