CST, 82168S, CD19 (1D3) Rat Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying CD19 mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD19 (1D3) Rat Monoclonal Antibody (PE Conjugate) (CST #82168) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.125 μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:160 Flow Cytometry (Live): 1:160 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD19 (1D3) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total mouse CD19 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5). The 1D3 clone is a widely used as a phenotypic marker for CD19 expression on the surface of murine B cells and subsets of dendritic cells. Alternate Names AW495831; B-lymphocyte antigen CD19; Cd19; CD19 antigen; Differentiation antigen CD19 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2a kappa