CST, 82196T, JAM-A (E6Z7E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JAM-A. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, JAM-A (E6Z7E) Rabbit Monoclonal Antibody (CST #82196) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity JAM-A (E6Z7E) Rabbit Monoclonal Antibody recognizes endogenous levels of total JAM-A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central sequence of human JAM-A protein. Background Junctional Adhesion Molecule-A/F11 Receptor (JAM-A/F11R) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. JAM-A regulates multiple cellular processes, including tight junction assembly, epithelial-mesenchymal transition (EMT), leukocyte migration, virus binding, platelet activation, and angiogenesis. Â Aberrant expression of JAM-A is correlated with poor patient prognosis in several human cancers (1,2). In a mouse model of atherosclerosis, an antagonistic peptide that inhibits JAM-A-expressing platelets from interacting with inflamed endothelial cells reduces atherosclerotic plaque formation (3). Alternate Names CD321; F11 receptor; F11R; JAM; JAM-1; JAM-A; JAM1; JAMA; JCAM; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG