CST, 82202S, Met (E4E4W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Met mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Met (E4E4W) Rabbit Monoclonal Antibody (CST #82202) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Met (E4E4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse Met protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular fragment containing IPT-domains of mouse Met protein. Background Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7). Alternate Names AI838057; c-Met; Hepatocyte growth factor receptor; HGF; HGF receptor; HGF/SF receptor; HGFR; Met; met proto-oncogene; met proto-oncogene tyrosine kinase; OTTMUSP00000028498; OTTMUSP00000028499; Par4; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 140, 170 Source/Isotype: Rabbit IgG