CST, 8226T, HIP2 (D27C4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HIP2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HIP2 (D27C4) Rabbit Monoclonal Antibody (CST #8226) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HIP2 (D27C4) Rabbit Monoclonal Antibody recognizes endogenous levels of total HIP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu161 of human HIP2 protein. Background Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). Huntingtin-interacting protein-2 (HIP2), also known as E2-25K, is a member of the E2 protein family that is highly expressed in the brain and catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-6). Recent studies have also implicated HIP2 in the control of apoptosis and cell-cycle progression through its ability to regulate the stability of Smac/Diablo and cyclin B1 (7,8). Alternate Names DKFZp564C1216; DKFZp686J24237; E2 ubiquitin-conjugating enzyme K; E2-25K; E2(25K); HIP-2; HIP2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UBC1; UBE2K; Ubiquitin carrier protein; ubiquitin conjugating enzyme E2 K; ubiquitin conjugating enzyme E2K; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); Ubiquitin-protein ligase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG