CST, 82306S, ZNF217 (E6X4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZNF217. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ZNF217 (E6X4R) Rabbit Monoclonal Antibody (CST #82306) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 29164 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity ZNF217 (E6X4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZNF217 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZNF217 protein. Background Zinc finger protein 217 (ZNF217) is an oncogenic transcription factor that regulates the expression of a wide variety of genes. ZNF217 is a member of the Kruppel-like factor (KLF) family of transcription factors, which consists of zinc finger proteins that bind to CACCC or GT box DNA elements (1). ZNF217 regulates the expression of its target genes through direct DNA binding, and through interaction with transcriptional co-repressors in the CoREST-HDAC complex (2). Although it was initially described as a transcriptional repressor, ZNF217 may also act as a transcriptional activator depending on the target gene (3). Amplification of the gene itself has been observed in a variety of tumors, where it is typically associated with poor prognosis (4-7). Overexpression of ZNF217 protein leads to increased cell proliferation and promotes tumor growth in breast and ovarian cancer (8,9). ZNF217 also interferes with apoptotic signaling and confers resistance to chemotherapeutic drugs in breast cancer (9,10). Alternate Names ZABC1; Zinc finger protein 217; ZN217; ZNF217 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG