CST, 82318S, BCKDK (E5B6J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCKDK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BCKDK (E5B6J) Rabbit Monoclonal Antibody (CST #82318) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BCKDK (E5B6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCKDK protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCKDK protein. Background Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Branched-chain α-keto acid dehydrogenase kinase (BCKDK) phosphorylates BCKDH-E1α (4). Phosphorylation of BCKDH-E1α plays a key role in regulating the enzymatic activity of this complex (3-6). Alternate Names [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; 3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial; BCKD; BCKD-kinase; BCKDHKIN; BCKDK; BCKDKD; BDK; branched chain alpha-ketoacid dehydrogenase kinase; branched chain keto acid dehydrogenase kinase; branched chain ketoacid dehydrogenase kinase; Branched-chain alpha-ketoacid dehydrogenase kinase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG