CST, 8231S, Homer1 Antibody

Polyclonal Antibody for studying HOMER1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Homer1 Antibody (CST #8231) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu130 of human Homer1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Homer1, Homer2 and Homer3 are scaffolding proteins, composed of an EVH protein-binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs PPXXF, PPXF, and LPSSP of G protein-coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (1-2). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. The Homer1 gene encodes a short isoform (Homer1a, aa 1-186) and two long isoforms (Homer1b, aa 1-354; Homer1c, aa 1-366). Homer1a lacks the coiled-coil domain and leucine zipper, antagonizing multimerization of Homers and thus disassembling signaling proteins complexes (3). Alternate Names HOME1; HOMER; homer homolog 1; homer homolog 1 (Drosophila); Homer protein homolog 1; homer scaffold protein 1; homer scaffolding protein 1; Homer-1; homer, neuronal immediate early gene, 1; HOMER1; HOMER1A; HOMER1B; HOMER1C; SYN47; Ves-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46 SOURCE: Rabbit