CST, 82342T, ARID2 (D8D8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARID2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ARID2 (D8D8U) Rabbit Monoclonal Antibody (CST #82342) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ARID2 (D8D8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val891 of human ARID2 protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). ARID2 is a unique member of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). ARID2 is the targeting subunit of the PBAF complex and critical for complex stability (14). It can also mediate DNA repair of double stranded breaks through interactions with RAD51 (15). ARID2 also has been demonstrated to be a tumor suppressor, and inactivating mutations have been found in various cancer types (16-18). Alternate Names ARID domain-containing protein 2; ARID2; AT rich interactive domain 2 (ARID, RFX-like); AT-rich interaction domain 2; AT-rich interactive domain-containing protein 2; BAF200; BRG1-associated factor 200; CSS6; DKFZp686G052; DKFZp779P0222; FLJ30619; KIAA1557; p200; Zinc finger protein with activation potential; zipzap; Zipzap/p200 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG