CST, 82389S, NUT (C52B1) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying NUT1. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, NUT (C52B1) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #82389) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity NUT (C52B1) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the human NUT protein. Background Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2). Alternate Names C15orf55; DKFZp434O192; FAM22H; MGC138683; MGC138684; Nuclear protein in testis; NUT; NUT family member 1; NUT midline carcinoma family member 1; NUTM1; Protein NUT Specification REACTIVITY: H R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG