CST, 8238S, p75NTR (D4B3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p75NTR. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, p75NTR (D4B3) Rabbit Monoclonal Antibody (CST #8238) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:1600 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Live): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46333 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live) Specificity / Sensitivity p75NTR (D4B3) Rabbit Monoclonal Antibody recognizes endogenous levels of total p75NTR protein. Nonspecific cytoplasmic staining is observed in fixed frozen mouse spleen and colon by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg198 of human p75NTR protein. This antibody is predicted to bind the extracellular amino-terminal region of p75NTR protein. Background The p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence, and a noncatalytic cytoplasmic domain (1). p75NTR displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell. Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway. In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2). The p75NTR protein undergoes sequential cleavage similar to APP and Notch. First, α-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling. At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions. γ-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4). Alternate Names CD271; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; nerve growth factor receptor; nerve growth factor receptor (TNFR superfamily, member 16); NGF receptor; NGFR; p75 ICD; p75(NTR); p75NTR; TNFR superfamily, member 16; TNFRSF16; TNR16; Tumor necrosis factor receptor superfamily member 16 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG