CST, 82503T, Phospho-Drebrin (Ser142) (F5H8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Drebrin (Ser142) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Drebrin (Ser142) (F5H8O) Rabbit Monoclonal Antibody (CST #82503) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Drebrin (Ser142) (F5H8O) Rabbit Monoclonal Antibody recognizes endogenous levels of Drebrin protein only when phosphorylated at Ser142. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 of human Drebrin protein. Background Developmentally-regulated brain proteins (Drebrins) are cytoplasmic proteins that were originally identified in the brain as F-actin-binding proteins. There are two mammalian isoforms: adult type (A) and embryonic type (E). These isoforms are derived from a single gene through alternative RNA splicing mechanisms (1). Drebrin E has been observed to accumulate in the developmental stage of migrating neurons and in the growing cell processes of neurons. Drebrin A is found at the dendritic spines of mature cortical neurons, where it plays a role in synaptic plasticity (2,3). Although Drebrins are primarily found in neurons, they have also been found in skeletal muscle, heart, pancreas, and kidney. Research studies have shown that reduced expression of Drebrin in the brain could be associated with Alzheimer's Disease, Down Syndrome (4), and bipolar disorders (5). Alternate Names D0S117E; DBN1; Developmentally-regulated brain protein; DKFZp434D064; DREB; Drebrin; drebrin 1; drebrin A; drebrin E; drebrin E2; drebrin-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110-132 Source/Isotype: Rabbit IgG