CST, 82504T, MDM2 (F7W7X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MDM2. Validated for WB,WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MDM2 (F7W7X) Rabbit Monoclonal Antibody (CST #82504) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62464 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity MDM2 (F7W7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDM2 protein. Based on the sequence of the protein antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoforms A1, B, and D. By western blot, this antibody detects a band of unknown origin at approximately 60 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MDM2 protein. Background MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). Alternate Names ACTFS; Double minute 2 protein; double minute 2, human homolog of; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; LSKB; MDM2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog (mouse); MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; ubiquitin-protein ligase E3 Mdm2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG