CST, 82517SF, RAR gamma1 (D3A4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying RARgamma. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, RAR gamma1 (D3A4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #82517) is ready to ship. Product Usage Information This product is the carrier free version of product #8965. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8965 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity RAR gamma1 (D3A4) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total RARγ1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with RARγ2. This antibody does not cross-react with either RARα or RARβ. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RARγ1 protein. Background Nuclear retinoic acid (RA) receptors (RARs) consist of three subtypes encoded by separate genes: α (NR1B1), β (NR1B2), and γ (NR1B3). For each subtype, there are at least two isoforms, which are generated by differential promoter usage and alternative splicing and differ only in their N-terminal regions. Retinoids, which are metabolites of vitamin A, serve as ligands for RARs (1). RARs function as ligand-dependent transcriptional regulators and are found to be heterodimerized with retinoid X receptors (RXRs). These transcriptionally active dimers regulate the expression of genes involved in cellular differentiation, proliferation, and apoptosis (2,3). Consequently, RARs play critical roles in a variety of biological processes, including development, reproduction, immunity, and organogenesis (4-6). RAR mutations, fusion proteins, altered expression levels, or aberrant post-translational modifications result in multiple diseases due to altered RAR function and disruption of homeostasis. In contrast to the ubiquitously expressed RARα subtype, RARγ displays a complex tissue-specific expression pattern (7). The hematopoietic system expresses significant levels of RARγ, and a recent study identified a role for RARγ in hematopoietic stem cell maintenance (8). RARγ is the predominant subtype in human and mouse epidermis, representing 90% of the RARs in this tissue (9-11). Given the high level of RARγ expression in the skin, it has been suggested that this nuclear receptor participates in a transcriptional program that governs maintenance and differentiation of normal epidermis and skin appendages. The transcriptional activity of RARγ is under stringent control, in part, through retinoic acid-induced phosphorylation and proteasomal degradation (12). Alternate Names NR1B3; Nuclear receptor subfamily 1 group B member 3; RAR-gamma; RARC; RARG; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2; Retinoic acid receptor gamma; retinoic acid receptor, gamma Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG